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violacein biosynthesis genes  (Addgene inc)


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    Addgene inc violacein biosynthesis genes
    Dynamic regulation of <t>biosynthesis</t> pathway with tryptophan-responsive MR-scRNAs. ( A ) Design schematic illustrating the dynamic regulatory strategy used to conditionally activate the <t>violacein</t> biosynthesis pathway in response to concentrations of the upstream metabolite, tryptophan, that is required for both growth and biochemical production. ( B ) Dose–response curve of the tryptophan MR-scRNA in response to the target metabolite. ( C ) Violacein titer extracted from E. coli cells with the violacein biosynthesis pathway activated by an on-target scRNA (+), non-targeting scRNA (−), or a tryptophan-responsive scRNA (MR-scRNA). Abbreviations: Ø, not detected; a.u., arbitrary units.
    Violacein Biosynthesis Genes, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/violacein+biosynthesis+genes/pETM6-G6-vioABE-4A6-vioC-3A2-vioD+(Plasmid+%2373440)/pmc12641264-36-0-6
    Average 93 stars, based on 2 article reviews
    violacein biosynthesis genes - by Bioz Stars, 2026-09
    93/100 stars

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    1) Product Images from "Metabolite-responsive scaffold RNAs for dynamic CRISPR transcriptional regulation"

    Article Title: Metabolite-responsive scaffold RNAs for dynamic CRISPR transcriptional regulation

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkaf1290

    Dynamic regulation of biosynthesis pathway with tryptophan-responsive MR-scRNAs. ( A ) Design schematic illustrating the dynamic regulatory strategy used to conditionally activate the violacein biosynthesis pathway in response to concentrations of the upstream metabolite, tryptophan, that is required for both growth and biochemical production. ( B ) Dose–response curve of the tryptophan MR-scRNA in response to the target metabolite. ( C ) Violacein titer extracted from E. coli cells with the violacein biosynthesis pathway activated by an on-target scRNA (+), non-targeting scRNA (−), or a tryptophan-responsive scRNA (MR-scRNA). Abbreviations: Ø, not detected; a.u., arbitrary units.
    Figure Legend Snippet: Dynamic regulation of biosynthesis pathway with tryptophan-responsive MR-scRNAs. ( A ) Design schematic illustrating the dynamic regulatory strategy used to conditionally activate the violacein biosynthesis pathway in response to concentrations of the upstream metabolite, tryptophan, that is required for both growth and biochemical production. ( B ) Dose–response curve of the tryptophan MR-scRNA in response to the target metabolite. ( C ) Violacein titer extracted from E. coli cells with the violacein biosynthesis pathway activated by an on-target scRNA (+), non-targeting scRNA (−), or a tryptophan-responsive scRNA (MR-scRNA). Abbreviations: Ø, not detected; a.u., arbitrary units.

    Techniques Used:

    Related Articles

    Plasmid Preparation:

    Article Title: Metabolite-responsive scaffold RNAs for dynamic CRISPR transcriptional regulation
    Article Snippet: Mammalian CRISPRa plasmids were derived from Addgene plasmid 84239 (a gift from Stanley Qi) [ ] or a commercial pcDNA3.1 vector. .. Violacein biosynthesis genes were obtained from Addgene plasmid 73440 (a gift from Mattheos Koffas) [ ]. ..



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    Figure 5. Targeted insertion of large fragments of DNA. (A) Schematic of the integration of 11.4 kb (CV 3274-CV 3270 from <t>Chromobacterium</t> violaceum ATCC <t>12472,</t> the violacein biosynthesis gene cluster), 22.6 kb (PA 4202-PA 4218 from Pseudomonas aeruginosa PAO1) or 31.7 kb (RS 08140-RS 08270 from Zymomonas mobilis ZM4) DNA fragment into site S3. (B) The patterns of panda and letters drawn on the plates using the strain MR-1 and MR- 1/10.4 kb. PCR products with multiple primers pairs probing for integration of (C) 11.4 kb, (D) 22.6 kb and (E) 31.7 kb into site S3, resolved by agarose gel electrophoresis. Control: the genome DNA from MR-1 was used as RCR template. (F) Integration efficiencies of 11.4, 22.6 and 31.7 kb DNA fragment at site S3. Data in (E) are shown as mean ± s.d. for n = 3 biologically independent samples.
    Kb Cv 3274 Cv 3270 From Chromobacterium Violaceum Atcc 12472 The Violacein Biosynthesis Gene Cluster, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/violacein+biosynthesis+genes/Chromobacterium+violaceum/pm35776123-169-14-21
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    violacein biosynthesis genes  (Addgene inc)
    93
    Addgene inc violacein biosynthesis genes
    Dynamic regulation of <t>biosynthesis</t> pathway with tryptophan-responsive MR-scRNAs. ( A ) Design schematic illustrating the dynamic regulatory strategy used to conditionally activate the <t>violacein</t> biosynthesis pathway in response to concentrations of the upstream metabolite, tryptophan, that is required for both growth and biochemical production. ( B ) Dose–response curve of the tryptophan MR-scRNA in response to the target metabolite. ( C ) Violacein titer extracted from E. coli cells with the violacein biosynthesis pathway activated by an on-target scRNA (+), non-targeting scRNA (−), or a tryptophan-responsive scRNA (MR-scRNA). Abbreviations: Ø, not detected; a.u., arbitrary units.
    Violacein Biosynthesis Genes, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/violacein+biosynthesis+genes/pETM6-G6-vioABE-4A6-vioC-3A2-vioD+(Plasmid+%2373440)/pmc12641264-36-0-6
    Average 93 stars, based on 1 article reviews
    violacein biosynthesis genes - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 5. Targeted insertion of large fragments of DNA. (A) Schematic of the integration of 11.4 kb (CV 3274-CV 3270 from Chromobacterium violaceum ATCC 12472, the violacein biosynthesis gene cluster), 22.6 kb (PA 4202-PA 4218 from Pseudomonas aeruginosa PAO1) or 31.7 kb (RS 08140-RS 08270 from Zymomonas mobilis ZM4) DNA fragment into site S3. (B) The patterns of panda and letters drawn on the plates using the strain MR-1 and MR- 1/10.4 kb. PCR products with multiple primers pairs probing for integration of (C) 11.4 kb, (D) 22.6 kb and (E) 31.7 kb into site S3, resolved by agarose gel electrophoresis. Control: the genome DNA from MR-1 was used as RCR template. (F) Integration efficiencies of 11.4, 22.6 and 31.7 kb DNA fragment at site S3. Data in (E) are shown as mean ± s.d. for n = 3 biologically independent samples.

    Journal: Nucleic acids research

    Article Title: Repurposing CRISPR RNA-guided integrases system for one-step, efficient genomic integration of ultra-long DNA sequences.

    doi: 10.1093/nar/gkac554

    Figure Lengend Snippet: Figure 5. Targeted insertion of large fragments of DNA. (A) Schematic of the integration of 11.4 kb (CV 3274-CV 3270 from Chromobacterium violaceum ATCC 12472, the violacein biosynthesis gene cluster), 22.6 kb (PA 4202-PA 4218 from Pseudomonas aeruginosa PAO1) or 31.7 kb (RS 08140-RS 08270 from Zymomonas mobilis ZM4) DNA fragment into site S3. (B) The patterns of panda and letters drawn on the plates using the strain MR-1 and MR- 1/10.4 kb. PCR products with multiple primers pairs probing for integration of (C) 11.4 kb, (D) 22.6 kb and (E) 31.7 kb into site S3, resolved by agarose gel electrophoresis. Control: the genome DNA from MR-1 was used as RCR template. (F) Integration efficiencies of 11.4, 22.6 and 31.7 kb DNA fragment at site S3. Data in (E) are shown as mean ± s.d. for n = 3 biologically independent samples.

    Article Snippet: Targeted insertion of large fragments of DNA. (A) Schematic of the integration of 11.4 kb (CV 3274-CV 3270 from Chromobacterium violaceum ATCC 12472, the violacein biosynthesis gene cluster), 22.6 kb (PA 4202-PA 4218 from Pseudomonas aeruginosa PAO1) or 31.7 kb (RS 08140-RS 08270 from Zymomonas mobilis ZM4) DNA fragment into site S3. (B) The patterns of panda and letters drawn on the plates using the strain MR-1 and MR1/10.4 kb.

    Techniques: Agarose Gel Electrophoresis, Control

    Dynamic regulation of biosynthesis pathway with tryptophan-responsive MR-scRNAs. ( A ) Design schematic illustrating the dynamic regulatory strategy used to conditionally activate the violacein biosynthesis pathway in response to concentrations of the upstream metabolite, tryptophan, that is required for both growth and biochemical production. ( B ) Dose–response curve of the tryptophan MR-scRNA in response to the target metabolite. ( C ) Violacein titer extracted from E. coli cells with the violacein biosynthesis pathway activated by an on-target scRNA (+), non-targeting scRNA (−), or a tryptophan-responsive scRNA (MR-scRNA). Abbreviations: Ø, not detected; a.u., arbitrary units.

    Journal: Nucleic Acids Research

    Article Title: Metabolite-responsive scaffold RNAs for dynamic CRISPR transcriptional regulation

    doi: 10.1093/nar/gkaf1290

    Figure Lengend Snippet: Dynamic regulation of biosynthesis pathway with tryptophan-responsive MR-scRNAs. ( A ) Design schematic illustrating the dynamic regulatory strategy used to conditionally activate the violacein biosynthesis pathway in response to concentrations of the upstream metabolite, tryptophan, that is required for both growth and biochemical production. ( B ) Dose–response curve of the tryptophan MR-scRNA in response to the target metabolite. ( C ) Violacein titer extracted from E. coli cells with the violacein biosynthesis pathway activated by an on-target scRNA (+), non-targeting scRNA (−), or a tryptophan-responsive scRNA (MR-scRNA). Abbreviations: Ø, not detected; a.u., arbitrary units.

    Article Snippet: Violacein biosynthesis genes were obtained from Addgene plasmid 73440 (a gift from Mattheos Koffas) [ ].

    Techniques: